Composite
Part:BBa_K2922043:Design
Designed by: Yuan Li Group: iGEM19_XMU-China (2019-10-13)
Expression system of DsbA-PbBD coding periplasm display of lead binding peptide with T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 328
Illegal NotI site found at 1472 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1385
Illegal BamHI site found at 521
Illegal XhoI site found at 883
Illegal XhoI site found at 1132 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 663
Illegal NgoMIV site found at 1588
Illegal NgoMIV site found at 2906 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 567
Design notes
We ordered the part DNA from Sangon ® company.
Source
DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.
Reference
1. C. Hui et al., Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice. Sci Rep 8, 5685 (2018).
2. C. Y. Hui, Y. Guo, X. Q. Yang, W. Zhang, X. Q. Huang, Surface display of metal binding domain derived from PbrR on Escherichia coli specifically increases lead(II) adsorption. Biotechnol Lett 40, 837-845 (2018).